goat anti mouse igs conjugated to allophycocyanin (SouthernBiotech)

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SouthernBiotech goat anti mouse igs conjugated to allophycocyanin
Goat Anti Mouse Igs Conjugated To Allophycocyanin, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 93/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+human+igm/Goat+Anti-Mouse+IgM%2C+Human+ads-APC/us12584929-471-37-47
Average 93 stars, based on 17 article reviews

goat anti mouse igs conjugated to allophycocyanin - by Bioz Stars, 2026-10

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Article Title: Exploring the standardization of human nasal antibody measurements
Article Snippet: Purified nasal sIgA, human serum, and purified serum IgG were used as WB standards.Purified nasal sIgA, human serum, and purified serum IgG were used as WB standards.. Horseradish peroxidase (HRP)-conjugated goat anti-human IgA heavy chain (HC) (Abcam, UK), goat anti-human IgG Fc (Abcam, UK), mouse anti-human IgM (SouthernBiotech, USA), goat anti-human IgE (Invitrogen, USA), and goat anti-human IgD heavy chain antibodies (GeneTex, USA), were used to detect IgA, IgG, IgM, IgE, IgD.. After adding chemiluminescent HRP substrate (Millipore, USA), polyvinylidene fluoride (PVDF) membranes were detected by Bio-Rad imaging system.After adding chemiluminescent HRP substrate (Millipore, USA), polyvinylidene fluoride (PVDF) membranes were detected by Bio-Rad imaging system.

Article Title: Exploring the standardization of human nasal antibody measurements.
Article Snippet: Purified nasal sIgA, human serum, and serum IgG were used as WB standards.Purified nasal sIgA, human serum, and serum IgG were used as WB standards.. Horseradish peroxidase (HRP)-conjugated goat anti-human IgA heavy chain (HC) (Abcam, UK), goat antihuman IgG Fc (Abcam, UK), mouse anti-human IgM (SouthernBiotech, USA), goat antihuman IgE (Invitrogen, USA), and goat anti-human IgD heavy chain antibodies (GeneTex, USA), were used to detect IgA, IgG, IgM, IgE, IgD.. After adding chemiluminescent HRP substrate (Millipore, USA), polyvinylidene fluoride (PVDF) membranes were developed by Bio-Rad imaging system.After adding chemiluminescent HRP substrate (Millipore, USA), polyvinylidene fluoride (PVDF) membranes were developed by Bio-Rad imaging system.

Article Title: Could Anti-retroviral treatment affects the profile of hepatitis B vaccine specific antibody in vertically HIV-infected children?
Article Snippet: The plates were washed five times as described above, then 100µl HRP-conjugated anti-human IgG (1:2000), HRP-conjugated anti-human IgG1, IgG2, IgG3, IgG4, or IgM (1:4000) antibodies were added in the appropriate plates and incubated for 1h at 37°C.The plates were washed five times as described above, then 100µl HRP-conjugated anti-human IgG (1:2000), HRP-conjugated anti-human IgG1, IgG2, IgG3, IgG4, or IgM (1:4000) antibodies were added in the appropriate plates and incubated for 1h at 37°C.. The secondary antibodies used in this study were mouse anti-human IgG Fc (clone JDC-10), mouse anti-human IgM (clone UHB), mouse anti-human IgG1 Fc (clone HP6001), mouse anti-human IgG2 Fc (clone 31-7-4), mouse anti-human IgG3 Hinge (clone HP6050) and mouse anti-human IgG4 pFc’ (clone HP6023), all HRP conjugated antibodies were procured from Southern Biotech® (Birmingham, USA).. Plates were washed five times and 100µl of 2,2′-Azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) substrate was added to each well and incubated in the dark for 30 min at 37°C.Plates were washed five times and 100µl of 2,2′-Azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) substrate was added to each well and incubated in the dark for 30 min at 37°C.

Article Title: Anti-human CD19 antibodies
Article Snippet: Human primary B cells are re-suspended at 1×106 cells/mL and cultured at 37° C. in polystyrene 96-well, u-bottom plates in complete medium (RPMI-1640 containing 10% Fetal bovine serum, 1×MEM-nonessential amino acids, 1 mM Sodium Pyruvate, 1× Penicillin-Streptomycin Solution (all from Corning) and 1× Glutamax (Gibco), 0.1% β-mercaptoethanol (Life Technologies).Human primary B cells are re-suspended at 1×106 cells/mL and cultured at 37° C. in polystyrene 96-well, u-bottom plates in complete medium (RPMI-1640 containing 10% Fetal bovine serum, 1×MEM-nonessential amino acids, 1 mM Sodium Pyruvate, 1× Penicillin-Streptomycin Solution (all from Corning) and 1× Glutamax (Gibco), 0.1% β-mercaptoethanol (Life Technologies).. Cells are pre-treated with anti-human CD19 antibody for 1 hour and stimulated with mouse anti-human IgM (2 μg/mL-Southern Biotech) plus rabbit anti-mouse IgG (12 μg/mL-Thermo Fisher) for 2 days at 37° and 5% CO2.. Cells are then pulsed with [3H]-thymidine (1 μCi thymidine/well, PerkinElmer, Boston, Mass.) for 18 hours of cell culture.Cells are then pulsed with [3H]-thymidine (1 μCi thymidine/well, PerkinElmer, Boston, Mass.) for 18 hours of cell culture.

Article Title: Depletion of Homeostatic Antibodies against Malondialdehyde-Modified Low-Density Lipoprotein Correlates with Adverse Events in Major Vascular Surgery
Article Snippet: Total IgG and IgM antibody levels were measured in sandwich format.Total IgG and IgM antibody levels were measured in sandwich format.. Goat anti-human IgG (1:800) or mouse anti-human IgM (1/400) (Southern Biotech, Birmingham, AL, USA) were used for capture.. Biotinylated goat F(ab’) 2 anti-human IgG (1/50,000) or biotinylated mouse anti-human IgM (1/15,000) (Southern Biotech, Birmingham, AL, USA), followed by HRP-conjugated streptavidin (1:200), were used for detection.Biotinylated goat F(ab’) 2 anti-human IgG (1/50,000) or biotinylated mouse anti-human IgM (1/15,000) (Southern Biotech, Birmingham, AL, USA), followed by HRP-conjugated streptavidin (1:200), were used for detection.

Article Title: Proinflammatory IgG Fc structures in patients with severe COVID-19
Article Snippet: Sera were diluted 5-fold starting at 1:50 and ELISAs were performed as described above.Sera were diluted 5-fold starting at 1:50 and ELISAs were performed as described above.. The various secondary antibodies used for isotyping were 1:5000 dilutions of HRP-conjugated Goat Anti-Human IgG Fc (Southern Biotech), Mouse Anti-Human IgM (Southern Biotech) and Goat Anti-Human IgA Fc (Southern Biotech).

Article Title: Oxidised LDL and Anti-Oxidised LDL Antibodies Are Reduced by Lipoprotein Apheresis in a Randomised Controlled Trial on Patients with Refractory Angina and Elevated Lipoprotein(a)
Article Snippet: Total IgG and IgM antibody levels were measured using capture ELISA.Total IgG and IgM antibody levels were measured using capture ELISA.. Goat anti-human IgG or mouse anti-human IgM (Southern Biotech, Birmingham, AL, USA) were used to capture IgG or IgM, respectively.. Detection antibodies were biotinylated goat F(ab′) ² anti-human IgG or biotinylated mouse anti-human IgM (both Southern Biotech).Detection antibodies were biotinylated goat F(ab′) ² anti-human IgG or biotinylated mouse anti-human IgM (both Southern Biotech).

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